CRL-1378 RBL-1 大鼠嗜碱性粒细胞白血病细胞， ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献优培养条件
CRL-1378 RBL-1 大鼠嗜碱性粒细胞白血病细胞 的详细介绍
CRL-1378
| ATCC® Number: | CRL-1378™ | Price: | $329.00 |
| Designations: | RBL-1 |
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| | Depositors: | H Metzger, C Isersky |
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| | Biosafety Level: | 1 |
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| | Shipped: | frozen |
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| | Medium & Serum: | See Propagation |
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| | Growth Properties: | suspension |
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| | Organism: | Rattus norvegicus （rat） |
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| | Morphology: | lymphoblast
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| | Source: | Organ: peripheral blood
Strain: Wistar
Disease: leukemia
Cell Type: basophil; chemically induced |
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| | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
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| | Applications: | transfection host （Nucleofection technology from Lonza） |
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| | Receptors: | FcERI （Fc of IgE） |
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| | Comments: | The line exhibits various characteristics of basophil differentiation including surface receptors for IgE. It was shown to not release histamine by an IgE mediated system. PubMed: 6166481 |
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| | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C |
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| | Subculturing: | Protocol: Cultures can be maintained by the addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 viable cells/ml and maintain between 1 X 10 exp5 cells/ml and 1 X 10 exp6 /ml..
Medium Renewal: Add medium as cell density increases |
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| | Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase |
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| | Related Products: | Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2003 recommended serum:ATCC 30-2020 |
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| | References: | 1232: Kulczycki A Jr., et al. The interaction of IgE with rat basophilic leukemia cells. I. Evidence for specific binding of IgE. J. Exp. Med. 139: 600-616, 1974. PubMed: 4812630 22475: Barsumian EL, et al. IgE-induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones. Eur. J. Immunol. 11: 317-323, 1981. PubMed: 6166481 22638: Eccleston E, et al. Basophilic leukaemia in the albino rat and a demonstration of the basopoietin. Nat. New Biol. 244: 73-76, 1973. |
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