CRL-1711 SF9 昆虫卵巢细胞

产品型号：
厂商性质：生产厂家
更新时间：2025-08-14
访问量：3125

产品咨询联系我们

产品分类

Product Category

细胞库

细胞原代牛、狗、鸡、鸭、鱼原代细胞羊原代细胞猪原代细胞兔原代细胞小鼠原代细胞大鼠原代细胞人原代细胞培养基原代细胞冻存液 ATCC 人乳腺癌猪正常细胞猪睾丸细胞系人胶质瘤细胞人成骨细胞人永生化肝细胞人肝癌细胞人卵巢癌细胞人结直肠腺癌人经典小细胞肺癌细胞人小细胞肺癌细胞人结直肠腺癌细胞人鼻腔上皮细胞中国仓鼠卵巢悬浮细胞嗜性逆转录病毒包装稳定细胞株小鼠神经细胞瘤人眼脉洛膜黑色素瘤 PT67 逆转录酶病毒包装细胞鸭子胚胎成纤维细胞猪肾细胞系昆虫细胞猫肾细胞恒河猴胚肾细胞仓鼠卵巢细胞负鼠肾细胞仓鼠肺细胞果蝇胚胎细胞家猫肺成纤维样细胞鸡胚细胞豚鼠心肌来源细胞豚鼠肺成纤维样细胞豚鼠皮肤成纤维样细胞狗肾细胞非洲绿猴胚胎肾上皮细胞猴胚胎肾上皮细胞长臂猿淋巴瘤新生牛眼囊成纤维细胞黑化大家鼠肺成纤维样细胞俄勒冈地鼠细胞家兔皮肤成纤维样细胞昆虫卵巢细胞人胸膜瘤细胞非洲绿猴肾细胞 VERO 衍生株（SVP）白化金黄地鼠肺成纤维样细胞黄胸鼠肺成纤维样细胞白鲢尾鳍细胞蝙蝠肺细胞草鱼肾细胞系大黄鱼肾细胞猴肾细胞系犬肾细胞系人结肠直肠腺癌猪肺泡巨噬细胞 PFHSA05（TLL2）人重组特洛德样蛋白因子 PFHSA04 （BMP1）人重组骨诱导因子人乳腺癌细胞结肠癌细胞成纤维细胞人胚肾细胞动物卵巢细胞动物胚胎细胞动物肺细胞肋骨来源细胞成纤维样细胞动物上皮细胞动物肝脏细胞可诱导表达稳定细胞株动物气管细胞动物肾细胞动物巨噬细胞人正常细胞人肿瘤细胞、癌细胞小鼠正常细胞小鼠肿瘤细胞、癌细胞大鼠正常细胞大鼠肿瘤细胞其它动物细胞查看全部产品

详细介绍

CRL-1711 SF9 昆虫卵巢细胞 的详细介绍

ATCC^® Number:

CRL-1711™

Price:

$275.00

Designations:	Sf9
Depositors:	G Smith, C Cherry, MD Summers
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	mixed: adherent/suspension
Organism:	Spodoptera frugiperda （fall armyworm）
Morphology:	epithelial
Source:	Organ: ovary
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation:	Isolation date: 1983
Applications:	transfection host （Roche FuGENE® Transfection Reagents technology from amaxa）
Virus Susceptibility:	Nuclear polyhedrosis virus, Autographa californica St. Louis encephalitis virus
Age:	pupa
Gender:	female
Comments:	This line can be used to replicate baculovirus expression vectors. It is important to use the medium described below.
Propagation:	ATCC complete growth medium: Grace's Insect Medium with L-glutamine and supplemented with: 500 mg/L calcium chloride 2800 mg/L potassium chloride 3330 mg/L lactalbumin hydrolysate 3330 mg/L yeastolate 10% Heat-inactivated fetal bovine serum （previously tested for insect cell culture） Atmosphere: air, 100% Temperature: 28.0°C Growth Conditions: The recommended media are formulated for use without CO2. Omission of the yeastolate or lactalbumin hydrolysate will lead to poor performance by this line.
Subculturing:	Protocol: Gently resuspend cells in the spent culture medium by pipetting across the monolayer or by hitting the flask against the palm of your hand （the latter is only preferable when working with larger flasks）. *Subction Ratio:** A subction ratio of 1:5 or greater is recommended Medium Renewal:* Every 2 to 3 days
Preservation:	Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
References:	22650: Vaughn JL, et al. The establishment of two cell lines from the insect Spodoptera frugiperda （Lepidoptera; Noctuidae）. In Vitro 13: 213-217, 1977. PubMed: 68913 23136: Zhang PF, et al. Susceptibility of the Sf9 insect cell line to infection with adventitious viruses. Biologicals 22: 205-213, 1994. PubMed: 7811453 23294: Smith GE, et al. Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector. Proc. Natl. Acad. Sci. USA 82: 8404-8408, 1985. PubMed: 3878519 32359: Hall MR, Gibson W. Cytomegalovirus assemblin: the amino and carboxyl domains of the proteinase form active enzym e when separay cloned and coexpressed in eukaryotic cells. J. Virol. 70: 5395-5404, 1996. PubMed: 8764050 32361: Bakker A, et al. Human T-cell leukemia virus type 2 Rex inhibits pre-mRNA splicing in vitro at an early stage of spliceosome formation. J. Virol. 70: 5511-5518, 1996. PubMed: 8764063 32693: Gibson W, et al. Cytomegalovirus "missing" capsid protein identified as heat-aggregable product of human cytomegalovirus UL46. J. Virol. 70: 7454-7461, 1996. PubMed: 8892863 32860: Filtz TM, et al. Purification and G protein subunit regulation of a phospholipase C-beta from Xenopus laevis oocytes. J. Biol. Chem. 49: 31121-31126, 1996. PubMed: 8940109 32882: Lindorfer MA, et al. G protein gamma subunits with altered prenylation sequences are properly modified when expressed in Sf9 cells. J. Biol. Chem. 271: 18582-18587, 1996. PubMed: 8702508 33023: Bruant SS, et al. N-terminal sequences contained in the Src homology 2 qand 3 domains of p120 GTPase-activating protein are required for full catalytic activity toward ras. J. Biol. Chem. 271: 5195-5199, 1996. PubMed: 8617802 33034: Stewart L, et al. Biochemical and biophysical analyses of recombinant forms of human topoisomerase I. J. Biol. Chem. 271: 7593-7601, 1996. PubMed: 8631793 33143: Kawabe J, et al. Soluble adenylyl cyclase from Spodoptera frugiperda （Sf9） cells. J. Biol. Chem. 271: 20132-20137, 1996. PubMed: 8702736 33160: Yasuda H, et al. Role of the prenyl group on the G protein gamma subunit in coupling trimeric G proteins to A1 adenosine receptors. J. Biol. Chem. 271: 18588-18595, 1996. PubMed: 8702509

产品咨询

上一篇：RabbitS1 家兔皮肤成纤维样细胞
下一篇：SMC-1 人胸膜瘤细胞

CRL-1711 SF9 昆虫卵巢细胞

留言框

产品：

您的单位：

您的姓名：

联系电话：

常用邮箱：

省份：

详细地址：

补充说明：

验证码：