CRL-2580 MR1 中国仓鼠X小鼠B淋巴细胞杂交瘤，ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献优培养条件
CRL-2580 MR1 中国仓鼠X小鼠B淋巴细胞杂交瘤 的详细介绍
| ATCC® Number: | CRL-2580™ | Price: | $323.00 |
| Designations: | MR1 [derivative of HB-11048] |
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| | Depositors: | Bristol-Myers Squibb Pharm. Res. Inst. |
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| | Isotype: | IgG |
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| | Biosafety Level: | 1 |
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| | Shipped: | frozen |
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| | Medium & Serum: | See Propagation |
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| | Organism: | Cricetulus migratorius （B cell）; Mus musculus （myeloma） （hamster, Armenian （B cell）; mouse （myeloma）） |
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| | Morphology: | lymphoblast
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| | Source: | Cell Type: hybridoma: B lymphocyte; |
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| | Cellular Products: | immunoglobulin; monoclonal antibody; against mouse CD40 ligand （CD154, CD40L, gp39） |
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| | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
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| | Comments: | Animals were immunized with mouse T cells （Th1）. Spleen cells were fused with NS-1 myeloma cells. The antibody detects a 39000 dalton protein selectively expressed on activated mouse T cells. A culture deposited at the ATCC as HB-11048 in May of 1992 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. |
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| | Propagation: | ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Temperature: 37.0°C |
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| | Subculturing: | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
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| | Preservation: | Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase |
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| | Related Products: | Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001 recommended serum:ATCC 30-2020 parental cell line:ATCC HB-11048 |
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| | References: | 23334: Noelle RJ, et al. A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells. Proc. Natl. Acad. Sci. USA 89: 6550-6554, 1992. PubMed: 1378631 23483: Roy M, et al. The regulation of the expression of gp39, the CD40 ligand, on normal and cloned CD4+ T cells. J. Immunol. 151: 2497-2510, 1993. PubMed: 8103067 |
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CRL-2580 MR1 中国仓鼠X小鼠B淋巴细胞杂交瘤
