CRL-2755 EMT6 大鼠乳腺癌细胞， ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献优培养条件
CRL-2755 EMT6 大鼠乳腺癌细胞 的详细介绍
CRL-2755
| ATCC® Number: | CRL-2755™ | Price: | $355.00 |
| Designations: | EMT6 |
|
| | Depositors: | S Rockwell |
|
| | Biosafety Level: | 1 |
|
| | Shipped: | frozen |
|
| | Medium & Serum: | See Propagation |
|
| | Growth Properties: | adherent |
|
| | Organism: | Mus musculus （mouse） |
|
| | Morphology: | epithelial
|
|
| | Source: | Organ: breast
Strain: BALB/cCrgl
Disease: mammary carcinoma
Cell Type: epithelial |
|
| | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
|
|
| | Isolation: | Isolation date: 1971 |
|
| | Tumorigenic: | Yes |
|
| | Gender: | female |
|
| | Comments: |
|
|
| | Subculturing: | Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days |
|
| | Preservation: | Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO
Storage temperature: liquid nitrogen vapor phase |
|
| | Related Products: | recommended serum:ATCC 30-2020 |
|
| | References: |
|
| |
