| Designations: | L-M（TK-） [LM（tk-）, LMTK-] |
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| Depositors: | TR Chen |
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| Biosafety Level: | 1 |
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| Shipped: | frozen |
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| Medium & Serum: | See Propagation |
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| Growth Properties: | adherent |
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| Organism: | Mus musculus （mouse） |
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| Morphology: | fibroblast
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| Source: | Tissue: subcutaneous connective tissue; areolar and adipose
Strain: C3H/An |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| CCL-1.3 L-M（TK-） 小鼠胸腺激酶缺陷细胞株 |
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| Applications: | transfection host （Roche FuGENE® Transfection Reagents） |
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| Tumorigenic: | Yes |
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| Antigen Expression: | H-2k |
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| Cytogenetic Analysis: | modal number = 45; range = 42 to 48. The stemline chromosome number is hyperdiploid with the 2S component occurring at 3.8%. Twenty-one markers were common to nearly all S metaphases studied. Of these, 13 were two-armed chromosomes, and eight, acrocentric. Four markers were isochromosomes by Robertsonian translocations from chromosomes 4, 12, 14 and 15. Normal X, 12, 14, 15 and 16 were absent. The X chromosome was present as an arm of Rb（X.3）. （See Cancer Genet. Cytogenet. 24: 95-107, 1987 for a detailed karyotype description.） |
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| Age: | 100 days |
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| Gender: | male |
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| Comments: | The cells are 5-bromo-2'-deoxyuridine resistant （BUDR, BrdUrd） and thymidine kinase （TK） deficient. HAT sensitive. Tested and found negative for ectromelia virus （mousepox）. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide （CO2）, 5% |
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| Subculturing: | Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week |
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| Preservation: | Freeze medium: complete growth medium, 95%; DMSO, 5% |
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| Related Products: | Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002 recommended serum:ATCC 30-2020 parental cell line:ATCC CCL-1 |
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| References: | 868: Kit S, et al. Deletion of thymidine kinase activity from L cells resistant to bromodeoxyuridine. Exp. Cell Res. 31: 297-312, 1963. PubMed: 14056989 21410: Chen TR. Cytogenetics of somatic cell hybrids. II. Modal karyotypes and mosaicism in mouse mutant cell lines: LM（tk-）, A9, and Rag. Cancer Genet. Cytogenet. 24: 95-107, 1987. PubMed: 3466673 25904: Hsu TC, Somers CE. Properties of L cells resistant to 5-bromodeoxyuridine. Exp. Cell Res. 26: 404-410, 1962. PubMed: 14449548 25907: Boone C, et al. Assignment of three human genes to chromosomes （LDH-A to 11, TK to 17, and IDH to 20） and evidence for translocation between human and mouse chromosomes in somatic cell hybrids （thymidine kinase-lactate dehydrogenase A-isocitrate dehydrogenase-C-11, E-17, and F-20 chromosomes）. Proc. Natl. Acad. Sci. USA 69: 510-514, 1972. PubMed: 4110482 25910: Peterson JL, McBride OW. Cotransfer of linked eukaryotic genes and efficient transfer of hypoxanthine phosphoribosyltransferase by DNA-mediated gene transfer. Proc. Natl. Acad. Sci. USA 77: 1583-1587, 1980. PubMed: 6929511 |
