immunoglobulin; monoclonal antibody; against human acetylcholinesterase
Permits/Forms:
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
AE-2 小鼠杂交瘤细胞抗AChE
Comments:
Animals were immunized with purified human erythrocyte acetylcholinesterase.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody cross-reacts with human and monkey neuromuscular junctions.
Tested and found negative for ectromelia virus （mousepox）.
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References:
939: Fambrough DM, et al. Acetylcholinesterase of human erythrocytes and neuromuscular junctions: homologies
