CMCC 28001 藤黄微球菌
冻干菌种的复苏培养：
1冻干管开封：用浸过75%酒精的脱脂棉擦净冻干管，用火焰加热其顶端，滴少量无菌水至加热顶端使之破裂，用锉刀或镊子敲下已破裂的冻干管顶端。
2菌种恢复培养：用无菌吸管 0.3-0.5毫升适宜到液体培养基，滴入冻干管内轻轻振荡，使冻干菌体溶解呈悬浮状，吸取全部菌体悬浮液，移植于1-2支建议的斜面培养基上，并在建议的温度下培养。
CMCC 28001 藤黄微球菌注意事项
1、微生物菌种应保藏于低温、清洁和干燥的地方，室温放置时间过长会导致菌种衰退；
2、菌种操作应在无菌条件下进行，防止杂菌污染；
3、斜面菌种保藏时间通常为3-6个月，应根据菌种状况及时转接；冻干菌种保藏时间通常为2-15年；
| Organism: | Escherichia coli (Migula) Caslani and Chalmers |
| Designations: | EDL 931 |
| Isolation: | feces, human |
| Depositor: | GK Morris |
| History: | ATCC <<--GK Morris<<--J.G. Wells |
| Biosafety Level: | 2 |
| Shipped: | freeze-dried |
| Growth Conditions: | ATCC medium18: Trypticase soy agar
Temperature: 37.0°C |
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| Antigenic Properties: | serotype O157:H7 |
| Cross References: | Nucleotide (GenBank) : AF305917Escherichia coli O157:H7 nonfunctional beta-glucuronidase (uidA) gene, complete cds. |
| Comments: | Hemorrhagic colitis [9698] Cytotoxic activity [8277] |
| References: | 8277: Beery JT, et al. Cytotoxic activity of Escherichia coli O157:H7 culture filtrate on the mouse colon and kidney. Curr. Microbiol. 11: 335-342, 1984.
9698: Riley LW, et al. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N. Engl. J. Med. 308: 681-685, 1983. PubMed: 6338386 |
