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ATCC 9027 铜绿假单胞菌 现货供应
菌 种 说 明
您好：
感谢您使用本单位的微生物菌种，我们有可用于科研、教学、生产的菌种约2500株，基本覆盖了环境、工业、农业、分析、食、药用真菌行业各类生产和科研用微生物，其中许多优良菌株的生产性能具有较高水平。我们对所提供菌种作如下说明：
菌种简介：
1、菌种名称：ATCC 9027 铜绿假单胞菌(绿脓杆菌)
2、规格：斜面□ 冻干管□
二、保藏条件：冻干菌种应在2-8℃保存；斜面菌种放15-20度保存，15-20天转接一次。
三、培养条件：
1. 培养基：营养琼脂培养基（NA）
胰蛋白栋大豆肉汤培养基（TSA）
LB培养基 以上培养基三选一即可
2. 培养温度： 37℃
3. 培养时间：18- 24h
四、真空冻干菌种的复苏培养：
1、安瓿管开封：用浸过75%酒精的脱脂棉擦净安瓿管，用火焰加热其顶端，滴少量无菌水至加热顶端使之破裂，用锉刀或镊子敲下已破裂的安瓿管顶端。
2、菌种恢复培养：用无菌吸管吸取 0.1-0.2毫升的培养液或无菌水，滴入安瓿管内轻轻振荡，使冻干菌体溶解呈悬浮状，吸取全部菌体悬浮液，移植于1-2支建议的斜面培养基上，并在建议的温度下培养。
五、注意事项：
1、微生物菌种应保藏于低温、清洁和干燥的地方，室温放置时间过长会导致菌种衰退；
2、菌种操作应在无菌条件下进行，防止杂菌污染；
3、冻干菌复苏后，应根据菌种状况及时转接传代
| ATCC® Number: | 9027™ | Price: | $155.00 |
| Organism:Pseudomonas aeruginosa （Schroeter） MigulaDesignations:R. Hugh 813Isolation:outer ear infectionDepositor:CP HegartyBiosafety Level:2Shipped:freeze-driedGrowth Conditions:ATCC medium3: Nutrient agar or nutrient brothTemperature: 37.0°CPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Applications:assay of [4101] assay of antimicrobial preservatives [11020] [21514] [21603] detection of [92787] efficacy testing [92779] media testing [11018] [11019] [21511] [92429] preparatory test control quality control strain [92096] [92409] sterility testing [21604] [92306] produces rhamnolipid surfactant [9924]Related Products:purified DNA: ATCC 9027D-5ATCC 9027 铜绿假单孢菌References:4101: ASTM International Standard Test Method for Preservatives in Water-Containing Cosmetics. West Conshohocken, PA9924: Zhang Y, Miller RM. Enhanced octadecane dispersion and biodegradation by a Pseudomonas rhamnolipid surfactant （biosurfactant）. Appl. Environ. Microbiol. 58: 3276-3282, 1992. PubMed: 144436311018: British Pharmacopoeia Commission Test for sterility. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI A, 200311019: British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 200311020: British Pharmacopoeia Commission Efficacy of antimicrobial preservation. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI C, 200321511: European Pharmacopoeia Commission Microbial contamination of products not required to comply with the test for sterility （tests for specified micro-organisms）. Nutritive and selective properties of the media and validity of the test for specified micro-organisms. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.6.13, 199721514: European Pharmacopoeia Commission Efficacy of antimicrobial preservation. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 5.1.3, 199721603: U.S. Pharmacopeia General Chapters:ANTIMICROBIAL EFFECTIVENESS TESTING. Rockville, MD:U.S. Pharmacopeia;USP USP28-NF23, 200521604: U.S. Pharmacopeia General Chapters:STERILITY TESTS. Rockville, MD:U.S. Pharmacopeia;USP USP28-NF23, 200532185: Jones CB, Platt JH. Propofol composition containing edetate. US Patent 5,714,520 dated Feb 3 199892096: Applied Biosystems MicroSeq Pharmaceutical Panel #1. :Applied Biosystems;MicroSeq92287: Efficacy of Preservation on Non-Eye Area Water-Miscible Cosmetic and Toiletry Formaulations. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 998.10.92304: Microbial limit test. Tokyo, Japan:Japanese Pharmacopoeia;JP Jp14e.part I.35.92306: Sterility test, Growth promotion test. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part I.54.92307: Preservatives-effectiveness test. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part II.12.92402: Ophthalmic optics--Contact lens care products--Microbiological requirements and test methods for products and regimens for hygienic management of contact lenses. Geneva （Switzerland）:International Organization for Standardization/ANSI;ISO ISO 14729:2001.92403: Ophthalmic optics--Contact lens care products--Antimicrobial preservative efficacy testing and guidance on determining discard date. Geneva （Switzerland）:International Organization for Standardization/ANSI;ISO ISO 14730:2000.92409: Applied Biosystems MicroSeq PHL Panel #1. :Applied Biosystems;MicroSeq92429: Medical microbiology-- Culture media -- Part 3: Dip slides for microbiological urine analysis. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58942-3: 2003, 200392779: Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria. London, UK:British Standards Institution;British Standard BS ISO 21149:2006.92787: Cosmetics - Microbiology - Detection of Pseudomonas aeruginosa. London, UK:British Standards Institution;British Standard BS ISO 22717:2006. | |||
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