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CCL-25 WISH 人羊膜细胞.
ATCC® Number: CCL-25™ Price: $323.00
Additional information about this cell line
Designations: WISH
Depositors: L Hayflick
Biosafety Level: 2
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens （human）
Morphology: epithelial
CCL-25 WISH 人羊膜细胞.
Source: Organ: HeLa contaminant
Cellular Products: keratin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Susceptibility: poliovirus 1, 2; adenovirus 3; vesicular stomatitis （Indiana）
Reverse Transcript: negative
DNA Profile （STR）: Amelogenin: X
CSF1PO: 9,10
D13S317: 13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Isoenzymes: G6PD, A
HeLa Markers: Y
Comments: This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
The cells are positive for keratin by immunoperoxidase staining.
NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination
Propagation: ATCC complete growth medium: Minimum essential medium （Eagle） in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:10 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and let culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22153: Hayflick L. The establishment of a line （WISH） of human amnion cells in continuous c*tion. Exp. Cell Res. 23: 14-20, 1961. PubMed: 13712490
26034: . . Am. J. Dis. Child. 103: 460, 1962.
CCL-25 WISH人羊膜细胞。
ATCC®编号：CCL-25™价格：323.00美元
有关此细胞系的其他信息
名称：WISH
存款人：L Hayflick
生物安全级别：2
运送：冷冻
中等和血清：见传播
生长特性：贴壁
生物体：智人（人）
形态：上皮
CCL-25 WISH
CCL-25 WISH人羊膜细胞。
来源：器官：HeLa污染物
蜂窝产品：角蛋白
许可证/表格：除上述MTA之外，转移此ATCC材料可能需要其他ATCC和/或管理许可证。购买ATCC材料的任何人终都需要负责获得许可。请点击此处了解有关装运到您所在地的具体要求的信息。
病毒易感性：脊髓灰质炎病毒1,2;腺病毒3;水泡性口炎（印第安纳州）
反转录：否定
DNA图谱（STR）：釉原蛋白：X.
CSF1PO：9,10
D13S317：13.3
D16S539：9,10
D5S818：11,12
D7S820：8,12
THO1：7
TPOX：8,12
vWA：16,18
同工酶：G6PD，A
HeLa标记：Y
评论：这条线
zi初被认为是源自正常的羊膜，但后来发现，基于同工酶分析，HeLa标记染色体和DNA指纹分析已经通过HeLa细胞污染建立。
通过免疫过氧化物酶染色，细胞对角蛋白呈阳性。
注意：此行的细胞含有HeLa标记染色体，并且是通过HeLa污染衍生的
繁殖：ATCC*生长培养基：Earle's BSS中的低必需培养基（Eagle），含有非必需氨基酸和1mM丙酮酸钠，90％;胎牛血清，10％
温度：37.0℃
传代培养：传代培养比例：建议传代比例为1：2至1:10
中等更新：每周两次
取出培养基，加入新鲜的0.25％胰蛋溶液3到5分钟，去除，让培养物在室温下静置5-10分钟。加入新鲜培养基，吸入并分装到新的培养瓶中。
保存：培养基95％; DMSO，5％
相关产品：推荐培养基（不含ATCC培养基描述的额外补充剂或血清）：ATCC 30-2003
推荐血清：ATCC 30-2020
参考文献：22153：Hayflick L.在连续培养中建立人羊膜细胞系（WISH）。进出口。细胞研究。 23：14-20,1961。PubMed：13712490
26034：。 。上午。 J. Dis。儿童。 103：460,1962。
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