HTB-38 HT-29 人结肠癌细胞

ATCC® Number:  HTB-38™

Designations:  HT-29

Depositors:

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: colon

Disease: colorectal adenocarcinoma

Cellular Products: secretory component of IgA; carcinoembryonic antigen (CEA); transforming growth factor beta binding protein; mucin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.

Isolation:  Isolation date: 1964

Applications: transfection host (Nucleofection technology from Lonza

Roche FuGENE® Transfection Reagents)

Receptors: human adrenergic alpha2A [23560]

urokinase receptor (u-PAR)

vitamin D (moderate expression)

urokinase receptor (u-PAR); vitamin D (moderate expression)

Virus Susceptibility: human immunodeficiency virus (HIV, LAV)

Tumorigenic: Yes

Oncogene: myc +; ras +; myb +; fos +; sis +; p53 +; abl -; ros -; src -

Antigen Expression: Blood Type A; Rh+; HLA A1, A3, B12, B17, Cw5

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 11,12

D16S539: 11,12

D5S818: 11,12

D7S820: 10

THO1: 6,9

TPOX: 8,9

vWA: 17,19

Cytogenetic Analysis: modal number = 71; range = 68 to 72.

The stemline chromosome number is hypertriploid with the 2S component occurring at 2.4%. Seventeen marker chromosomes are found in most metaphases, generally in single copy per chromosome. The marker designations are: M1p-(=t(3p-;?) with a deleted short arm), t(7q;?), t(10q;?), i(13q), 19q+a; M6, ?t(8q;9q-), ?Xp, M9, 6q+, t(13;?)a, t(13;?)b, 19q+b, M14, M15, 15p+, and Xq-. Chromosome 13 is nullisomic and chromosomes 8 and 14 are generally monosomic. No Y chromosome was detected by QM band analysis.

Isoenzymes:  AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 1-2

PGM3, 1-2

Age:  44 years adult

Gender:  female

Ethnicity:  Caucasian

Comments: Ultrastructural features reported for HT-29 cells include microvilli, microfilaments, large vacuolated mitochondria with dark granules, smooth and rough endoplasmic reticulum with free ribosomes, lipid droplets, few primary and many secondary lysosomes. The cells express urokinase receptors, but do not have detectable plasminogen activator activity [PubMed ID: 8381394]. HT-29 cells are negative for CD4, but there is cell surface expression of galactose ceramide (a possible alternative receptor for HIV). The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes. The p53 antigen is overproduced, and there is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution. N-myc oncogene expression was not detected.

There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation:  Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007

recommended serum:ATCC 30-2020

derivative:ATCC CCL-218

purified DNA:ATCC HTB-38D

HTB-38 HT-29 人结肠癌细胞

MLE-12 小鼠肺上皮细胞  

saos-2 人成骨肉瘤细胞

MA104 非洲绿猴胚胎肾上皮细胞

ball-1 细胞

nci-h226 人肺鳞癌细胞

MCA38 小鼠结肠癌细胞

LTPA 小鼠胰腺癌细胞

NCI-H23 人非小细胞肺癌细胞

293.00     人胚肾细胞

293FT    人胚肾细胞-用于慢病毒包装

293TN    人胚肾细胞--用于慢病毒包装

293AV    人胚肾细胞-用于腺病毒包装

293T    人胚肾细胞

208F    大鼠成纤维细胞

786-O    人肾透明细胞腺癌细胞

A172    人脑胶质母细胞瘤细胞

A549    人肺癌细胞 细胞系购买

AGS    人胃癌细胞

BEL7402 人肝癌细胞系

AZ-521    人胃癌细胞

B16F0    小鼠黑色素瘤细胞

Bel-7402    人肝癌细胞

Bel-7404    人肝癌细胞

BGC-823    人胃癌细胞

C6    大鼠脑胶质瘤细胞

SW480    人结肠腺癌细胞

CaCo-2    人结肠癌细胞

Cho-K1    仓鼠卵巢细胞亚株

COS-7    非洲绿猴SV40转化的肾细胞

GES-1    人胃上皮细胞

HCT-8    人回盲肠癌细胞

HeLa

Hepa1-6    小鼠肝癌细胞

HepG2    人肝癌细胞

hFOB1.19    人永生化成骨细胞

HGC-27    人胃癌细胞

Hs 746T    人胃癌细胞

HT-29    人结肠癌细胞

KB    人口腔表皮样癌细胞

LoVo    人结肠癌细胞

LLC-PK1    猪肾近曲小管上皮细胞

MCF-10A    人正常乳腺上皮细胞

MCF-7    人乳腺癌细胞

MDA-MB-231    人乳腺癌细胞

MGC80-3    人胃癌细胞

MKN-45    人胃癌细胞

NCI-H460    人大细胞肺癌细胞

NIH-3T3    小鼠成纤维细胞

NUGC-3    人胃癌细胞

Raw264-7    小鼠单核细胞白血病细胞

SGC-7901    人胃癌细胞

SMMC-7721    人肝癌细胞

SNU-638    人胃癌细胞

SNU-739    人肝癌细胞

Tca8113    人舌鳞癌细胞    细胞系购买

U251    人脑胶质瘤细胞

U-373MG    人脑神经胶质瘤细胞

U-87 MG    人脑星形胶质母细胞瘤

BT-474    人乳腺导管癌细胞

PANC-1    人胰腺癌细胞

HEK293    人胚肾细胞

Hep3B    人肝癌细胞

HL-7702    人正常肝细胞

Raw-Blue    小鼠单核细胞白血病细胞

WEHI-231    小鼠B淋巴细胞

OPM-2    人骨髓瘤细胞

NCI-H929    人骨髓瘤细胞

U266    人骨髓瘤细胞

EJ    人膀胱癌细胞

HOC    大鼠肝卵圆细胞

RWPE-1    人正常前列腺上皮细胞

CAL 27    人舌鳞癌细胞

Calu-3    人肺腺癌细胞

Eca-109    人食管癌细胞

DLD-1    人结直肠腺癌上皮细胞

5637.00     人膀胱癌细胞

SK-OV-3    人卵巢癌细胞

GBC-SD    人胆囊癌细胞

BEL7402 人肝癌细胞系

SW620    人结肠癌细胞

MRC-5    人胚肺成纤维细胞     细胞系购买

PC9    人肺癌细胞

PC14    人肺癌细胞

BxPC    人原位胰腺腺癌细胞

PK（15）    猪肾上皮细胞

MDCK    狗肾上皮细胞

3D4/21    猪肺泡巨噬细胞

MHCC-97L    人肝癌细胞（低转移）

MHCC-97H    人肝癌细胞系（高转移）

MiaCaPa-2    人胰腺癌细胞

HN13    口腔鳞状细胞癌细胞

Bend3    小鼠脑微血管内皮细胞

QBC939    人胆管癌细胞

Ishikawa    人子宫内膜癌细胞

A498    人肾癌细胞

143B

WEHI-231    小鼠B淋巴细胞

BN

P9R    小鼠骨髓基质细胞

P9    小鼠骨髓基质细胞

F98    大鼠脑胶质瘤细胞

NCIH1299    人非小细胞肺癌细胞

NCIH1650-M3    人非小细胞肺癌细胞

293LVT    人胚肾细胞--用于慢病毒包装

EC9706    人食管癌细胞

CNE    人鼻咽癌细胞

H322    人非小细胞肺癌细胞

Panc O2

S8    BEL7402 人肝癌细胞系

R5    细胞系购买

Pig MSC    猪骨髓间质干细胞

HCAEC    人冠状动脉内皮细胞

marc-145    猴胚胎肾上皮细胞

3D4/21    猪肺泡巨噬细胞

PK（15）    猪肾上皮细胞

MADB-106    大鼠乳腺癌细胞

Sf9    昆虫卵巢细胞

DADY

HUVEC[HUV-EC-C]    人脐静脉内皮细胞

PC14    人肺癌细胞

PC9    人肺癌细胞

Eca-109    人食管癌细胞

CAL 27    人舌鳞癌细胞

HOC    肝卵圆细胞

EJ    人膀胱癌细胞

U266    人骨髓瘤细胞

293-mTLR5    人胚肾细胞

Raw-Blue    小鼠单核细胞白血病细胞

HL-7702    人正常肝细胞

Hep3B    人肝癌细胞

U-373MG    人脑神经胶质瘤细胞

Tca-8113    人舌鳞癌细胞

SNU-739    人肝癌细胞

SNU-638    人胃癌细胞

NUGC-3    人胃癌细胞

NIH-3T3    小鼠成纤维细胞

MKN-45    人胃癌细胞

LLC-PK1    猪肾近曲小管上皮细胞

Hs 746T    人胃癌细胞

Hepa1-6    小鼠肝癌细胞

HCT-8    人回盲肠癌细胞

BEL7402 人肝癌细胞系

HaCaT    人永生化表皮细胞

GES-1    人胃上皮细胞

B16-F0    小鼠黑色素瘤细胞

AZ-521    人胃癌细胞

786-O    人肾透明细胞腺癌细胞

208F    大鼠成纤维细胞

293TN    人胚肾细胞--用于慢病毒包装

293FT    人胚肾细胞-用于慢病毒包装

GP-293    人胚肾细胞

293-L.P.    人胚肾细胞